Identification of Excretory Secretory (ES) Liquid Antigen Protein Fasciola gigantica with Polyethilen Glycol (PEG) Separation
نویسندگان
چکیده
منابع مشابه
Determination of somatic and excretory-secretory antigens of Fasciola hepatica and Fasciola gigantica using SDS-PAGE
Human fascioliasis due to unknown species and animal fascioliasis caused by both or one of Fasciola spp. are commonly seen in Iran. To compare electrophoretic patterns of somatic and excretory-secretory antigens of F. hepatica and F. gigantica by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), the adult flukes were collected from infected slaughtered bovine livers. E/S a...
متن کاملImmunolocalization and immunodetection of the excretory/secretory (ES) antigens of Fasciola gigantica
The digenetic trematode Fasciola gigantica is a parasite of great agricultural and economic importance. Along with Fasciola hepatica, F. gigantica incurs huge economic losses to the agricultural sector. Because of unavailability of an effective and commercial vaccine, the earliest diagnosis of the disease is the only way to control the disease. The conventional coprological techniques are able ...
متن کاملEffect of Fasciola gigantica excretory secretory antigen on rat hematological indices
The present study was undertaken to investigate the effect of Fasciola gigantica excretory secretory antigen (Fg-ESA) on rat hematological indices. Fg-ESA was prepared by keeping thoroughly washed 40 F. gigantica flukes in 100 ml phosphate buffer saline (PBS) for 2 h at 37 degrees C, and centrifuging the supernatant at 12,000 g at 4 degrees C for 30 min. The protein content of Fg-ESA was adjust...
متن کاملDevelopment and characterization of monoclonal antibodies against excretory-secretory antigens of Fasciola gigantica.
Monoclonal antibodies (MAbs) directed against Fasciola gigantica excretory-secretory (ES) antigens were developed from BALB/c mice. Four were selected for further study, from the panel of hybridomas. The antigen specificities of these MAbs were characterized and localized by enzyme-linked immunoeletrotransfer blot (EITB) and immunoperoxidase technique. The target epitopes of these MAbs are 66 k...
متن کاملdetermination of somatic and excretory-secretory antigens of fasciola hepatica and fasciola gigantica using sds-page
human fascioliasis due to unknown species and animal fascioliasis caused by both or one of fasciola spp. are commonly seen in iran. to compare electrophoretic patterns of somatic and excretory-secretory antigens of f. hepatica and f. gigantica by sodium dodecyl sulphate polyacrylamide gel electrophoresis (sds-page), the adult flukes were collected from infected slaughtered bovine livers. e/s a...
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ژورنال
عنوان ژورنال: Indonesian Journal of Biotechnology
سال: 2017
ISSN: 2089-2241,0853-8654
DOI: 10.22146/ijbiotech.27017